Afucosylated monoclonal antibodies are monoclonal antibodies engineered so that the oligosaccharides in the Fc region of the antibody do not have any fucose sugar units. When antibodies are afucosylated, antibody-dependent cellular cytotoxicity (ADCC) is increased.

This review discusses the relevance of antibody core fucosylation to ADCC, different strategies to produce afucosylated antibodies, and an update of afucosylated antibody drugs currently undergoing clinical trials as well as those that have been approved.

2024年6月19日 · Afucosylated antibodies are characterized by the absence of fucose sugar residues on their Fc (fragment crystallizable) region. This modification has major implications for the antibody's functionality, particularly in enhancing antibody-dependent cellular …

Afucosylation is a central procedure to alter the composition of antibodies by lowering their fucose levels. This, on the other hand, has far-reaching consequences on their characteristics and possible applications. One key focus is afucosylated antibodies and their role in immunotherapy.

2024年10月29日 · Afucosylation is an advanced glycoengineering technique for therapeutic antibodies to optimize immune responses, particularly antibody-dependent cellular cytotoxicity (ADCC).

IgG is normally fully fucosylated, and afucosylated IgG is only raised against antigens associated with human cells, thus introducing a mechanism for fine-tuning IgG potency through increased affinity for FcγRIII.

2023年5月18日 · Afucosylated immunoglobulins have been shown to enhance certain viral infections. Here, the authors generate and structurally characterize a unique nanobody that inhibits these pathogenic...

Afucosylatedantigen-speci ficIgG leads to immunopathology in SARS-CoV-2 and dengue virus infections, and en-hanced severity in alloimmunity (preg-nancy and transplantation), while correlating with control of HIV-1 infec-tions and protective traits against malaria.

2023年11月30日 · Afucosylation of antibodies offers distinct advantages, primarily enhancing their ability to engage with the immune system. By removing fucose, afucosylated antibodies exhibit heightened antibody-dependent cellular cytotoxicity (ADCC).

This review discusses the relevance of antibody core fucosylation to ADCC, different strategies to produce afucosylated antibodies, and an update of afucosylated antibody drugs currently undergoing clinical trials as well as those that have been approved.

2023年4月7日 · Core fucosylation is a highly prevalent and significant feature of N -glycosylation in therapeutic monoclonal antibodies produced by mammalian cells where its absence (afucosylation) plays a key role in treatment safety and efficacy.

2022年8月22日 · Afucosylated antigen-specific IgG leads to immunopathology in SARS-CoV-2 and dengue virus infections, and enhanced severity in alloimmunity (pregnancy and transplantation), while correlating with control of HIV-1 infections and …

2022年1月18日 · In two prospective cohorts, early non-neutralizing, afucosylated immunoglobulin G (IgG) antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were associated with progression from mild to more severe COVID-19.

2020年12月23日 · In this study, we investigated the induction of afucosylated IgG to various antigens and delineated its dynamics and proinflammatory potential in COVID-19. Afucosylated IgG responses have only been found in various alloimmune responses against cellular blood groups and two enveloped viruses.

Core fucosylation is a highly prevalent and significant feature of N-glycosylation in therapeutic monoclonal antibodies produced by mammalian cells where its absence (afucosylation) plays a key role in treatment safety and efficacy.

This scientific article focuses on the superior antibody expression platform, FucoFree system, for the custom expression of afucosylated mAbs with enhanced antibody-dependent cellular cytotoxicity (ADCC).

2023年3月3日 · The enhanced effect by afucosylated PD-L1 antibody was dependent on neutrophils and associated with decreased frequencies of PD-L1 + myeloid cells and increased infiltration of T cells in the TME. Our data reveal that the current design of FDA-approved anti–PD-L1 mAbs does not optimally harness FcγR pathways and suggest two strategies to ...

2022年10月1日 · Afucosylated antigen-specific IgG leads to immunopathology in SARS-CoV-2 and dengue virus infections, and enhanced severity in alloimmunity (pregnancy and transplantation), while correlating with control of HIV-1 infections and …

2022年10月25日 · Sino Biological has developed a proprietary “FucoFree” eukaryotic cell culture technology for the custom expression of afucosylated mAbs. The company offers >95% purity, high throughput, high...