We’ve mastered DNA synthesis so you can focus on your next genomics breakthrough. Our custom primers and easy-to-use primer design tools help you save valuable time without sacrificing quality.

Order IDT's single-stranded, duplexed, and pooled DNA oligos. Design oligos and PCR primers with quenchers, fluorophores, spacers, linkers, and modifications.

ReadyMade Primers are stocked oligonucleotides for sample preparation, PCR, sequencing, and gene expression analysis of common genes. Each primer contains 10 μg of HPLC-purified product. Identity is confirmed by mass spectrometry* …

2024年1月30日 · To improve the success of your qPCR experiment it is necessary to design good primers that are complementary to the nucleotide sequence of interest. You will need two primers, one forward and one reverse, to amplify the target DNA. You want to design exon-exon primers since they are specific for amplifying cDNA, not genomic DNA (gDNA) (Figure 1).

IDT offers several free online tools for qPCR primer and probe design. The PrimeTime™ Predesigned qPCR Assays Design Tool , PrimerQuest™ Tool , OligoAnalyzer™ Tool , and RealTime PCR Tool guide you through identifying primer and probe sequences to make sure they are specific to your assay.

2023年9月5日 · The PrimerQuest™ Tool offers you the option to customize almost 50 different parameters that will lead to the most efficient primer design for your experiment. These customizable parameters let you select primer, probe, and amplicon criteria to target regions. Refer to this article for more information on the PrimerQuest™ Tool.

Meet IDT. We’re a global genomics advocate, creating custom solutions for research in CRISPR, NGS, qPCR, synthetic biology, and more.

PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR ® Green (Thermo Fisher Scientific) or EvaGreen ® (Biotium) dyes. Predesigned sequences for human, mouse, or rat are designed with advanced bioinformatic and thermodynamic sequence analytics and for easy selection.

2013年10月21日 · For PCR primer design, IDT recommends that you aim for PCR primers between 18 and 30 bases; however, the most important considerations for primer design should be the T m value and on-target binding efficiency. Primers should also be free of strong secondary structures and self-complementarity.

Adapters & indexing primers are key components of an NGS library prep workflow. Explore the xGen NGS Adapters & Indexing Primers for Illumina sequencing.