ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility. [1] In 2013, the technique was first described as an alternative advanced method …

2021年2月9日 · The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research.

The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression.

Genome-wide mapping of insertion ends by high-throughput sequencing allows for multidimensional assays of the regulatory landscape of chromatin with a rel-atively simple protocol that can be carried out in hours for a standard sample size of 50,000 cells.

2020年2月3日 · Here, we discuss the major steps in ATAC-seq data analysis, including pre-analysis (quality check and alignment), core analysis (peak calling), and advanced analysis (peak differential analysis and annotation, motif enrichment, …

ATAC-seq identifies open chromatin regions, ChIP-seq verifies and accurately locates transcription factor binding sites, and RNA-seq evaluates changes in gene expression. Such detailed analyses can illuminate the connections between chromatin accessibility, transcription factor interaction, and downstream target gene regulation, providing ...

A recommended practical technique for studying the openness of chromatin is the assay for transposase-accessible chromatin with high throughput sequencing (ATAC-Seq). The idea is to employ the transposase Tn5 to quickly connect to open chromatin, but Tn5 lacks the capability to …

In this chapter, we focus on chromatin accessibility data measured by the Single-Cell Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (scATAC-seq) or as part of the 10x Multiome assay (scATAC combined with scRNA-seq).

2019年8月15日 · Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) is an effective way to reveal chromatin accessibility at a genome-wide level.

Explore ATAC-seq, a pivotal tool for epigenetic analysis, unraveling chromatin dynamics and gene regulation in eukaryotic cells. Understand its advantages, limitations, and applications for high-throughput studies.