Here, we show that a short incubation in nonionic aqueous solutions at temperatures above 70 degrees C can efficiently break the interaction without denaturing the streptavidin tetramer. …

The affinity of streptavidin for biotin is the strongest non-covalent biological interaction known, with a dissociation constant (Kd) in the region of 10-15M. Each streptavidin monomer can bind one …

The capture of the small molecule biotin (vitamin H/vitamin B 7) by the bacterial protein SA (streptavidin) is both a powerful tool in biology and a model system for the study of high-affinity …

Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules, as shown in the diagram below, making this …

Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7 or vitamin H). With a dissociation constant (K d) on the order of ≈10 −14 mol/L, [1] the …

The effect of biotin binding on streptavidin (STV) structure and stability was studied using differential scanning calorimetry, Fourier transform infrared spectroscopy (FT-IR), and …

2019年12月18日 · Elevated biotin in the plasma due to its very high uptake interferes with diagnostic assays that use biotin–streptavidin technology for clinical analytes and biomarkers, …