In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. [1] A branched DNA assay begins with a dish or some other solid support (e.g., a plastic dipstick).

Providing a reliable means for direct quantification of viral load in clinical specimens, bDNA assays have been developed to measure hepatitis B virus DNA, hepatitis C virus RNA), human immunodeficiency virus type 1 RNA, and cytomegalovirus DNA.

Branched DNA assay uses nonenzymatic means to amplify the signal from HIV RNA. In this assay, viral RNA is ‘captured’ by hybridization to complementary oligonucleotides that are bound to the wells of a microtiter plate.

QuantiGene Assays incorporate branched DNA technology for accurate gene expression profiling. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal amplification rather than target amplification.

2021年6月1日 · Branched DNA (bDNA) allows the design and synthesis of signal amplification assays to detect nucleic acid sequences in hybridization assays quantitatively. Examples are assays for the detection of early viral infection states in plasma.

The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological...

QuantiGene Plex Assays incorporate branched DNA technology, which allows for the direct measurement of RNA transcripts by using signal amplification rather than template amplification. The assays are simple and easy to use; QuantiGene Plex Assays do not require RNA purification, cDNA synthesis, or PCR amplification.

The branched DNA (bDNA) assay represents a significant advance in the direct quantification of nucleic acid molecules for research and clinical applications.

In biology, a branched DNA assay is a test for specific nucleic acid chains, and is typically used to detect retroviruses such as HIV.[1] However, the assay can be used to detect and quantitate other types of RNA or DNA target. In the assay, branched DNA is mixed with a sample to be tested.

One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection.