You can prepare the stock solution of cellulase in either citrate buffer or acetate buffer (pH range 4.5-5.5) and store it at 4 degree Celsius in cold room. This can easily serve your purpose for...

Cellulase complexes with β-glucosidase for degrading cellobiose to glucose polymer. The lignin content in straw and furfural residues hampers cellulase production.

I have powdered cellulase from aspergillus niger (sigma cat: C1184-5KU). How do I make a stock solution of this enzyme, and what is the best temperature for long term storage of the stock...

Cellulase (EC 3.2.1.4; systematic name 4-β-D-glucan 4-glucanohydrolase) is any of several enzymes produced chiefly by fungi, bacteria, ... The reaction is terminated by adding an alkaline solution to stop enzyme activity and the reaction slurry is filtered or centrifuged. The colour in the filtrate or supernatant is measured and can be related ...

I have purchased a solid cellulase from Aspergillus niger (CAS Number 9012-54-8). I want to use it in solution as a positive control for CMC breakdown using agarose plate assays (incubate and...

Cellulases are enzymes that hydrolyse cellulose and related cellu-oligosaccharides derivatives. Its applications are enormous but high cost of production is the bottle-neck against the utilization of cellulase in industries.

Dissolve 1 g cellulase in 99 ml sterile distilled water. Filter sterilize through a 0.45 µm filter. Cellulase solution may be stored at 2-5°C for 2 weeks. Adapted from Chapter 5,...

To standardize an enzymatic assay procedure of cellulase. 2. Scope. This procedure applies to all cellulase and hemicellulase products. 3. Definitions. 3.1 Method: Spectrophotometric Stop Rate Determination. 3.2 Conditions: T = 37 °C, pH = 5.0, Abs340nm, Light Path = 1 cm. 3.3 ATP = Adenosine 5’-Triphosphate. 3.4 ADP = Adenosine 5’-Diphosphate

Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost.

Presently cellulosic ethanol is used as an additive in gasoline. This experiment demonstrates the effectiveness of cellulase, an enzyme that breaks down cellulose in paper pulp.