2016年7月3日 · Be suspicious: Never trust that any culture is what it says on the label, even newly bought cells. Be cautious: Only buy from reputable cell banks. Quarantine incoming samples until their identity can be confirmed and their cleanliness status verified.

This tutorial will help explain the basics of monitoring your cells, including what a healthy culture looks like, cell confluency, and the different phases of cell culture growth. This video will also show common indicators of bacterial and mycoplasma contamination.

Culture contaminants may be biological or chemical, seen or unseen, destructive or seem-ingly benign, but in all cases they adversely affect both the use of your cell cultures and the quality of your research. Contamination problems can be divided into three classes:

Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or …

Gain essential knowledge and practical tips to prevent cell culture contamination in your lab. Learn how to detect and treat common biological contaminants.

When working with sensitive cell lines like HeLa cells, any compromise in technique can lead to rapid contamination spread. Key prevention measures include: Regular cleaning and decontamination of work surfaces; Proper personal protective equipment usage; Sterile handling techniques for all cell culture procedures

2024年12月1日 · Circular contamination in cell culture refers to the introduction of unwanted elements—microorganisms, chemicals, or even foreign cells—into the culture system. This type of contamination disrupts the controlled environment needed for cell growth, often leading to compromised results and wasted resources. Contamination can manifest in various ways.

2022年11月1日 · Contamination is a constant risk in every cell culture laboratory. And while the threat of contamination will always remain, good working practices and proactive screening are essential to reduce the likelihood and severity of outbreaks—protecting your …

In this article, you will learn about what kinds of contamination to expect in your daily lab work and what you can do to prevent them. Contaminations by bacteria, fungi, and yeasts are among the most frequent in cell cultures, as they surround us everywhere and at all times.

In this article, we'll go over how to identify and handle cells with bacterial, fungal, or microbial contamination, mycoplasma contamination, cell line cross-contamination, and virus or pathogen contamination.