As iPSCs serve tremendous correction potentials for various diseases, we highlight the successes and challenges of iPSCs in cell replacement therapy and the synergy of iPSCs and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene …

2024年4月26日 · In this review, we begin by outlining the historical development of the iPSC technology, including the key discoveries that led to the breakthrough of somatic cell reprogramming to iPSCs in...

Learn how to reprogram induced pluripotent stem cells (iPSCs). iPSCs can be induced by forcing somatic cells to express transcription factors associated with pluripotency eg Oct4, Klf4, Sox2, cMyc, etc (Takahashi et al., 2006).

2017年5月22日 · Reprogramming somatic cells into induced pluripotent stem cells (iPSCs), which possess unique properties of self-renewal and differentiation into multiple cell lineages, is achieved by...

Using a single transfection of self-replicating RNA can produce high numbers of integration-free iPSCs following a 3-4 week protocol. Determine the optimal plating density of target human fibroblast cells (SCC058).

Gene reprogramming of induced pluripotent stem cells (iPSCs) refers to the reprogramming of somatic cells, such as skin cells or blood cells, into stem cells with the potential for multidirectional differentiation by introducing specific transcription factors.

The iPSC reprogramming process involves introducing specific transcription factors into somatic/ adult stem cells, which induces epigenetic changes and reprograms them into a pluripotent state capable of differentiating into various cell types. Key factors in reprogramming (Yamanaka factors)

Gene editing services using CRISPR/Cas9 technology for SNP replacement, large reporter gene knock-in, and DNA knock-out in iPSC and other cell lines. Our experts have extensive experience and can work with in-house or provided cell lines.

2011年2月22日 · Chromatin remodelling is a rate-limiting step during iPSC generation 42, and chemical compounds that alter DNA methylation or chromatin modifications improve reprogramming in various cell types.

Using iPSC technology, center researchers have reprogrammed skin cells into active motor neurons, egg and sperm precursors, liver cells, bone precursors, and blood cells.