Visualizing Mitochondrial Form and Function within the Cell
For light microscopy, there are three general approaches to visualizing mitochondrial structures: 1) labeling mitochondria in live cells with fluorescent dyes, 2) immunostaining fixed cells with mitochondria-specific fluorescent antibodies, and 3) using genetically encoded fluorescent mitochondrial tags. Using live cell fluorescent dyes to ...
Mitochondria under the microscope — Science Learning Hub
2012年2月29日 · Transmission electron microscopy (left) shows the complex internal membrane structure of mitochondria, and electron tomography (right) gives a three-dimensional view. Microscopes have been crucial for our understanding of mitochondrial structure and function. Mitochondria are visible under the light microscope although little detail can be seen.
Light microscopy of mitochondria at the nanoscale - PMC
In this review, we provide an overview on recent studies using super-resolution microscopy to investigate mitochondria, discuss the strengths and opportunities of the various methods to address specific questions in mitochondrial biology and highlight potential future developments.
Light Microscopy of Mitochondria at the Nanoscale
In this review, we provide an overview of recent studies using super-resolution microscopy to investigate mitochondria, discuss the strengths and opportunities of the various methods in addressing specific questions in mitochondrial biology, …
Visualizing mitochondrial dynamics at the nanoscale
2024年9月9日 · In this issue, Ren et al. introduce HBmito Crimson, a fluorogenic and photostable mitochondrial probe for STED microscopy and investigate how mitochondrial dynamics influence the spatial...
Super-resolution microscopy of mitochondria - ScienceDirect
2014年6月1日 · STED super-resolution microscopy and electron microscopy were used to determine the submitochondrial localization of the MICOS (MINOS, MitOS) complex in mitochondria of primary fibroblasts. The study revealed a regularly spaced pattern of MICOS clusters arranged in parallel to the cell growth surfaces in peripheral mitochondria.
Methods to Study Mitochondrial Structure and Function
2012年10月11日 · High- and super-resolution fluorescent microscopy has been used to visualize mitochondria and measure number of mitochondrial characteristics. Electron microscopy and electron tomography can visualize not only mitochondrial ultrastructure but also mitochondrial multiprotein complexes at near-atomic resolution.
Insight into mitochondrial structure and function from electron ...
2002年9月10日 · Although electron microscopy of conventionally prepared thin sections of cells and tissues has provided a wealth of information on the ultrastructure of mitochondria, deducing the three-dimensional structure of a complex object from individual thin slices can be perilous.
A semi-automatic method for extracting mitochondrial cristae ...
2024年3月28日 · Here, we present a semi-automatic method for the segmentation, 3D reconstruction, and shape analysis of mitochondria, cristae, and intracristal spaces based on 2D EM images of the murine...
Long-term super-resolution inner mitochondrial membrane
2023年10月19日 · A lipid-like small molecule, MAO-N3, was developed to assemble inner mitochondrial membrane-specific probes for confocal and various super-resolution microscopy techniques, with significantly ...