To investigate the roles of motors in mitosis, we treated S2 cells with unique motor protein dsRNA and cultured them for 3–4 d without Con A, then adhered them to Con A–coated coverslips, and examined the mitotic spindle and chromosomes by anti …

At least seven families of kinesin-related motor proteins have been localized to the mitotic spindle in vertebrate cells. In the budding yeast S. cerevisiae, five such motor proteins have been shown to work together in the spindle.

The orthologous protein in yeast, Kar3, preferentially heterodimerizes with either Vik1p or Cik1p, although it can homodimerize as well. Structural studies have revealed these proteins to be kinesin motor domains that lack an ATP-binding site yet still bind microtubules.

Sep 15, 2003 · Using RNA interference (RNAi) to deplete individual (or combinations of) motors followed by immunofluorescence and time-lapse microscopy, we have examined the mitotic functions of cytoplasmic dynein and all 25 kinesins in Drosophila S2 cells.

Motor proteins and their tracks are key regulators of mitochondrial homeostasis, and in this Review, we discuss the diverse functions of the three classes of motor proteins associated with mitochondria – the actin-based myosins, as well as the microtubule-based kinesins and dynein.

Microtubules are responsible for a variety of cell movements, including the intracellular transport and positioning of membrane vesicles and organelles, the separation of chromosomes at mitosis, and the beating of cilia and flagella.

Sep 7, 2000 · The mitotic spindle uses microtubule-based motor proteins to assemble itself and to segregate sister chromatids. It is becoming clear that motors invoke several distinct mechanisms to...

Aug 18, 2000 · In addition, microtubule-based motor proteins provide essential forces for microtubule organization and chromosome movement. Motors found localized to the spindle include cytoplasmic dynein and seven different families of proteins related to …

Jan 1, 1995 · Motor Proteins in Mitosis and Meiosis domain of variable length that separates the motor domain from a highly divergent globular domain. The purpose of this nonconserved domain is believed to provide the ability to recognize a broad variety of …

Sep 15, 2003 · Using RNA interference (RNAi) to deplete individual (or combinations of) motors followed by immunofluorescence and time-lapse microscopy, we have examined the mitotic functions of cytoplasmic dynein and all 25 kinesins in Drosophila S2 cells.