2021年7月16日 · Using intravital imaging of mouse GCs and single-cell RNA sequencing, Jacobsen et al. report that T follicular helper (T FH) cells are a critical player in this process. They found that some late-GC T FH cells upregulate the transcription factor FOXP3 and acquire a regulatory T cell–like phenotype.

2014年3月17日 · Here, we test the role of FoxP3 + regulatory T cells (Tregs) in controlling T follicular helper (Tfh) and germinal centre (GC) B-cell responses to influenza. In contrast to the idea that Tregs...

2011年7月24日 · Follicular helper (T FH) cells provide crucial signals to germinal center B cells undergoing somatic hypermutation and selection that results in affinity maturation. Tight control of T FH...

2015年9月14日 · Here we test the role of FoxP3 + regulatory T cells (Tregs) in controlling T follicular helper (Tfh) and germinal-center (GC) B cell responses to influenza. In contrast to the idea that Tregs suppress T cell responses, we find that Treg depletion severely reduces the Tfh cell response to influenza virus.

In this chapter, we describe how Foxp3 (+) follicular regulatory T (Tfr) cells, a population of regulatory T (Treg) cells related to T follicular helper (Tfh) cells and involved in the regulation of germinal centers (GC), can be identified by flow cytometry. Flow cytometry is a technology that allows multiparametric analysis of individual cells.

4 天之前 · CD4+ T follicular helper (TFH) cells are essential for orchestrating robust humoral immunity, yet the signals that initiate TFH cell differentiation are not fully understood. ... Notably, PbTII cells do not adopt features of FOXP3 + T regs (fig. S3A). ... Elevated IL-7R (figs. S7, A and B and S8A) and IL-2R (fig. S9, A and B) were confirmed via ...

2021年7月16日 · We show that contraction of immunization-induced GCs is immediately preceded by an acute surge in GC-resident Foxp3 + T cells, attributed at least partly to up-regulation of the transcription factor Foxp3 by T follicular helper (T FH) cells.

In this chapter, we describe how Foxp3 + follicular regulatory T (Tfr) cells, a population of regulatory T (Treg) cells related to T follicular helper (Tfh) cells and involved in the regulation of germinal centers (GC), can be identified by flow cytometry.

This chapter will provide an explanation of the cell preparation process for detecting Tfh cells via flow cytometry by combining a panel of extracellular and intracellular markers that have been used across multiple settings to delineate and characterize this CD4 + T cell subset.

2024年12月1日 · CD4 + CXCR5 + TFH, CD4 + CXCR5 + PD-1 + TFH, CD4 + CXCR5 + Foxp3 + follicular regulatory T cells (TFR), and desialylated platelets in peripheral blood were measured by flow cytometry. Plasma cytokines were assessed by enzyme-linked immunosorbent assay.