SOLVED: BIOCHEMISTRY LABORATORY #9 BICINCHONIC ACID (BCA) PROTEIN ASSAYThe BCA assay is another assay used to determine the concentration of protein in solutions. It is moderately sensitive, being slightly less sensitive than the Lowry Assay. It is related to the Lowry and Biuret assays in that it uses copper ion as its primary reagent. In the BCA assay, Trp, Tyr, and Cys residues in protein reduce Cu2 to Cu . The Cu ion forms a complex with bicinchonic acid which absorbs at 480 and 562 nm. We

Protein Quantification (BCA Assay)

BCA Assay for Protein Determination